Conclusion The method established to extract DNA applies to genotype the mtDNA CA dinucleotide repeats in decomposed tissue and keratinized tissue and is also a useful maker in forensic genetics. 结论用本文建立的方法提取DNA适用于腐败及角化组织的mtDNA(CA)n重复子分型检验,在法医学上具有一定的应用价值。
Objective To establish a genotyping method of the mitchondrial ( mtDNA) CA dinucleotide repeats in decomposed tissue and keratinized tissue. 目的建立腐败及角化组织的mtDNA(CA)n重复子分型方法。
Light microscope after pathological see: a slight thickening of gingival keratinized epithelium, epithelial spikes extended subepithelial dense connective tissue collagen fibers arranged in bundles of coarse, there are few capillaries and inflammatory cell infiltration. 术后病理切片光镜下观察见:牙龈角化上皮略有增厚,上皮钉突延长,上皮下结缔组织中致密粗大的胶原纤维排列成束,内有少量毛细血管和炎细胞浸润。